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RP-HPLC peptydów i białek

RP-HPLC peptydów i białek

Jelena Golushko / Oksana Rotkaja / Peteris Mekss

58,96 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Química
ISBN:
9786209363283
58,96 €
IVA incluido
Disponible
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Rozdzielanie związków wielkocząsteczkowych jest bardzo trudne, o ile w ogóle możliwe, w warunkach izokratycznych i wymaga elucji gradientowej. Teoria elucji gradientowej dla małych cząsteczek jest dobrze ugruntowana, jednak jej zastosowanie do separacji gradientowej biopolimerów w fazie odwróconej nie jest proste ze względu na specyficzne problemy, takie jak powolna dyfuzja, ograniczona dostępność fazy stacjonarnej dla większych cząsteczek lub możliwe zmiany konformacyjne próbki podczas elucji. Wysokosprawna chromatografia cieczowa została wykorzystana do zbadania zachowania różnych białek w chromatografii odwróconych faz. Wykorzystując układ woda/rozpuszczalnik organiczny/kwas trifluorooctowy, zbadano wpływ parametrów eksperymentalnych; wyniki chromatograficzne uzyskane dla różnych nośników faz stacjonarnych były porównywalne.

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